When running the given commands in Python interactive mode, the output will be 'ac'. The expression 'a = 5' and 'b = 10' assign integer values to the variables 'a' and 'b'. However, these assignments do not affect the concatenation operation 'd = "a" + "c"'. Here, 'a' and 'c' are string literals, and the '+' operator concatenates them, resulting in the string 'ac'. Therefore, the 'print(d)' statement will output 'ac'.