To add multiple accounts from a file using PVWA bulk-upload, the minimum permissions required are 'add accounts' to create the new accounts, 'update account content' to input or modify the information within the accounts themselves, and 'update account properties' to modify the metadata or attributes associated with the accounts. Permissions like 'rename accounts', 'view safe members', and 'add safes' are not directly relevant to the process of bulk-uploading accounts.