When an Amazon S3 gateway endpoint is configured in a VPC, the private subnets within the VPC need to have their route tables updated to route the S3 traffic to the gateway endpoint, instead of the internet.
Without the appropriate route table updates, the EC2 instance in the private subnet will not be able to communicate with the S3 bucket, even if the instance has the necessary S3 permissions.
Option A is incorrect because the issue is not related to the IAM role permissions, but rather the routing configuration.
Option B is incorrect because allowing outbound traffic to 0.0.0.0/0 on port 80 is not necessary to solve this problem. The issue is with the routing to the S3 gateway endpoint, not the internet access.
Option D is incorrect because the S3 bucket policy is not the issue here. The problem is with the routing, not the permissions on the S3 bucket.